IAE OpenIR  > 沈阳应用生态研究所
A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars
He, HB; Xie, HT; Zhang, XD; Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
2006
Source PublicationSOIL BIOLOGY & BIOCHEMISTRY
ISSN0038-0717
Volume38Issue:5Pages:1083-1091
AbstractAmino sugars have been used as biomarker to indicate microorganism contribution to soil organic matter turnover and sequestration. However, there is no direct gas chromatograph mass spectrometry (GUMS) approach to assess microbial synthesis of amino sugars in soil. We developed a novel method which combines laboratory incubation of substrate containing N-15 or C-13 and a GUMS technique to trace N-15 or C-13 isotope changes in three amino sugars, glucosamine, galactosamine, and muramic acid. Sample preparation followed the procedure of Zhang and Amelung (1996) [Zhang, X., Amelung, W., 1996. Gas chromatographic determination of muramic acid, glucosamine, galactosamine, and mannosamine in soils. Soil Biology and Biochemistry 28, 1201-1206.] The GUMS determination was conducted using a full scan mode with both electronic ionization (EI) and chemical ionization (0) sources. The CI source was suitable for all of the three amino sugars, while the El source was not applicable to muramic acid due to its low sensitivity in the determination as well as low concentration of muramic acid in soil. The enrichment of 15N or 13C in amino sugars during incubation was estimated by calculating the atom percentage excess (APE). 15N incorporation was evaluated according to fragment (F) abundance ratio of mass F + 1 to F, whilst C-13 incorporation was estimated according to the ratio of mass F + n to F (n is skeleton carbon number in the fragment). This novel method was assessed by using two soil samples (a Kandiudult and a Udoll) incubated with either N-15-amonium or U-C-13-glucose. The results indicate that the GUMS determination is reproducible, thus this technique is useful in detecting the microbial synthesis of amino sugars in soil, and especially it should be possible when looking at the position or how much labeled carbon and nitrogen atoms have been incorporated. (c) 2005 Elsevier Ltd. All rights reserved.
KeywordAmino Sugar C-13 N-15 Incubation Gc/ms Atom Percentage Excess (Ape)
Subject AreaSoil Science
Indexed BySCI
Language英语
WOS IDWOS:000237545900025
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.72.129.5/handle/321005/54964
Collection沈阳应用生态研究所
Corresponding AuthorZhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
Recommended Citation
GB/T 7714
He, HB,Xie, HT,Zhang, XD,et al. A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars[J]. SOIL BIOLOGY & BIOCHEMISTRY,2006,38(5):1083-1091.
APA He, HB,Xie, HT,Zhang, XD,&Zhang, XD, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China.(2006).A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars.SOIL BIOLOGY & BIOCHEMISTRY,38(5),1083-1091.
MLA He, HB,et al."A novel GUMS technique to assess N-15 and C-13 incorporation into soil amino sugars".SOIL BIOLOGY & BIOCHEMISTRY 38.5(2006):1083-1091.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[He, HB]'s Articles
[Xie, HT]'s Articles
[Zhang, XD]'s Articles
Baidu academic
Similar articles in Baidu academic
[He, HB]'s Articles
[Xie, HT]'s Articles
[Zhang, XD]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[He, HB]'s Articles
[Xie, HT]'s Articles
[Zhang, XD]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.